Effect of Secretome-Hypoxia Mesenchymal Stem Cells on Regulating SOD and MMP-1 mRNA Expressions in Skin Hyperpigmentation Rats

BACKGROUND: Ultraviolet B (UVB) radiation is the main factor causing hyperpigmentation. MSC secretome contains bioactive soluble molecules such as cytokines and growth factors that can accelerate skin regeneration. However, molecular role of in hyperpigmentation still unclear. AIM: This study aimed to determine effect hypoxia mesenchymal stem cells (S-HMSC) gel on expression superoxide dismutase (SOD) matrix metalloproteinases (MMP-1) genes tissue hyperpigmented rats induced by UVB light exposure. MATERIALS AND METHODS: Experimental research with post-test only control group. The control, base gel, T1 T2 groups were irradiated 6 times 14 days at 302 nm an minimal erythema dose 390 mJ/cm2, respectively, while sham group did not receive was given 100 uL S-HMSC 200 every day for days, received gel. On 15, isolated analyzed SOD MMP-1 using qRT-PCR. RESULTS: relative gene treatment (P1 = 0.47 ± 0.20, P2 1.22 0.47) increased increasing compared (UVB 0.05 0.01, Base 0.02). (T1 5.82 1.16, 2.86 1.57) decreased (Control 10.10 2.31, 9.55 1.29). CONCLUSION: Administration increase decrease model light.